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Histopathological effects of Algan hemostatic agent (AHA) in liver injury model in rats
1Department of Histology and Embryology, Bahcesehir University School of Medicine, Istanbul, Turkey
2Department of Medicine, Bahcesehir University School of Medicine, 2nd Grade Student, Istanbul, Turkey
3Department of General Surgery, Maltepe University School of Medicine, Istanbul, Turkey
4Department of Biostatistics, Mustafa Kemal University School of Medicine, Hatay, Turkey
Hepatology Forum 2022; 1(3): 16-20 DOI: 10.14744/hf.2021.2021.0040
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Abstract

Background and Aim: In this study, we aimed to assess the hemostatic and histopathological impacts of the Algan hemostatic agent (AHA) with the liver injury model.
Material and Methods: 24 male rats, 10-12 week old, were randomly divided into three equal groups (n=8) as control (physiological saline solution), AHA liquid and AHA powder. A total of three iatrogenic cut injuries were performed on the anterior surface of the left liver lobe. After bleeding started, sponges soaked with physiological saline, AHA liquid, AHA powder were gently pressed on the injured area for 20 seconds in corresponding groups, respectively. The bleeding time was measured with a timer. Failure to stop bleeding after three consecutive applications was considered as a failure. Animals were euthanized at the tenth minute of the procedure. Left liver lobes were removed for histopathological examination.
Results: Bleeding control success rates of AHA liquid were significantly higher than that of the AHA powder group, and both forms were more effective than physiological saline. A superficial thick granulation tissue with entrapped powder residual materials was detected in the AHA powder group. Liver parenchyma was intact in liquid and powder groups.
Conclusion: AHA is a fast-acting and applicable hemostatic agent in the liver bleeding model. However, further comparative studies in various organs are needed.