Amin Bagheri1 2Department of Pharmacognosy and Medicinal Plants Reasearch Center, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran
3Department of Pathology, Dr Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
4Firoozgar Neurology Department , Iran University of Medical Science, Tehran , Iran
5Tehran Heart Center, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Cardiac Surgery, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, IranTehran Heart Center, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Background and Aim: This study aims to demonstrate the hemostatic ef-fect of the hydroalcoholic extract of Achillea millefolium L. in localized bleeding and to assess the safety of its topical application on rat liver.
Material and Methods: The aerial parts of A. millefolium were macerated in methanol for two days. Twelve female Wistar rats, weighing 120–220 g, underwent anesthesia and laparotomy. The liver was exposed, and two incisions were made to induce bleeding. One incision was treated with a sponge soaked in A. millefolium extract, while the other served as a control. The animals were divided into two groups: in one, A. millefolium (150 mg/kg) was applied to the first incision, and in the other, to the second incision. Liver biopsies were collected after 4, 6, and 8 weeks.
Results: Application of A. millefolium to liver incisions, whether first or second, significantly reduced bleeding time (by 36.1% and 31.9%, respec-tively). Histopathological analysis showed no signs of toxicity or hepatic damage after 4, 6, and 8 weeks in the female rats.
Conclusion: The study confirms the hemostatic effect of the hydroalcoholic extract of A. millefolium in localized bleeding and establishes its safety for topical use.
