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Bioinformatic Analysis of Genetic Changes CLOCK, BMAL1, CRY1, CRY2, PER1, PER2, PER3 and NPAS2 Proteins in HCC Patients
1Department of Medical Biochemistry, Nigde Training and Research Hospital, Nigde, Turkiye
2Department of Medical Biochemistry, Nigde Omer Halisdemir University, School of Medicine, Nigde, Turkiye; 3Nigde Training and Research Hospital, Department of Gastroenterology, Nigde, Turkiye
Hepatology Forum 2023; 4(3): 108-117 DOI: 10.14744/hf.2023.2023.0009 PMCID: PMC10564247
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Background and Aim: Genes related to the circadian rhythm control vari-ous biological processes. The aim of this study was to comprehensively in-vestigate the mutational and mRNA profile of core circadian rhythm genes in hepatocellular cancer (HCC) samples.
Material and Methods: In this study, the gene profile of a total of 369 patients with HCC was examined over the data obtained from the cancer genome atlas database through-cBioPortal. The effects of mutations on protein were examined by scoring the Polymorphism Phenotyping v2, Mu-tation Assessor, and SIFT-databases. While the association of genes with other genes was determined with the GeneMANIA-database, the associa-tion of expression levels in the genes with overall survival (OS) was evalu-ated with the Kaplan–Meier Plot database.
Results: As a result of the analyses, there were a total of 25 mutations. De-creased expression levels of PER1 (1.3e-05), PER3 (p=0.046), and CRY2 (p=1.8e-06) genes were found statistically associated with shorter OS. It was also found that increased expression levels of the PER2 (p=0.045) gene were associated with longer OS, and increased expression levels of the NPAS2 (p=9e-04) gene were associated with shorter OS.
Conclusion: In particular, changes in the PER1, PER2, CRY2, and NPAS2 genes may provide possible molecular targets in chemotherapy and im-munotherapy for HCC patients.